All lanes : Anti-Huntingtin antibody (ab155942) at 1/500 dilutionLane 1 : -Lane 2 : +C2Lane 3 : +C3Lane 4 : +C6Lane 5 : +C7Lane 6 : Huntingtin23Q(1-513)Lane 7 : Huntingtin23Q(1-536)Lane 8 : Huntingtin23Q(1-552)Lane 9 : Huntingtin23Q(1-586)Lysates/proteins at 20 µg per lane.
Sandwich Elisa analysis of 293T cells (overexpressing Huntingtin neo epitope 552), labeling Huntingtin protein (0, 0.5,1,1.5 and 2µg) with ab155942 at 1/20 dilution.
Immunofluorescent analysis of 293T cells (transfected with Huntingtin23Q and Huntingtin148Q stop constructs ending in amino acid 552), labeling Huntingtin ab155942 at 1/50 dilution.
Immunohistochemistry analysis of Mouse cortex tissue samples, labeling Huntingtin with ab155942 at 1/50 dilution (green). Blue: Tissue counterstained with nuclei stain.