Anti-Huntingtin antibody [EP867Y]
| Name | Anti-Huntingtin antibody [EP867Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab45169 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP867Y |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues specific to the apopain cleavage site of human Huntingtin |
| Description | Rabbit Monoclonal |
| Gene | HTT |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Huntingtin antibody [EP867Y] (ab45169) at 1/10000 dilution + SH-SY-5Y cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5783_ab45169_1.jpg)
Anti-Huntingtin antibody [EP867Y] (ab45169) at 1/10000 dilution + SH-SY-5Y cell lysate
Ab45169 staining human Huntingtin in human brain tissue by immunohistochemistry using paraffin embedded tissue.Ab45169 staining human Huntingtin in human brain tissue by immunohistochemistry using paraffin embedded tissue.
Overlay histogram showing SH-SY5Y cells stained with ab45169 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45169, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
ICC/IF image of ab45169 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45169, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Expression of genes encoding the calcium signalosome in cellular and transgenic - Expression of genes encoding the calcium signalosome in cellular and transgenic
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Inhibition of apoptosis signal-regulating kinase 1 reduces endoplasmic reticulum - Inhibition of apoptosis signal-regulating kinase 1 reduces endoplasmic reticulum
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