Anti-Huntingtin antibody [EPR5526]

Name	Anti-Huntingtin antibody [EPR5526]
Supplier	Abcam
Catalog	ab109115
Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR5526
Applications	WB IHC-P ICC/IF
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide, corresponding to residues in the N terminus of Human Huntingtin
Description	Rabbit Monoclonal
Gene	HTT
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Huntingtin antibody [EPR5526] (ab109115) at 1/10000 dilutionLane 1 : SH-SY5Y cell lysateLane 2 : HeLa cell lysateLane 3 : U87-MG cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

ab109115, at 1/100, staining Huntingtin in paraffin embedded Human brain tissue by Immunohistochemistry.

ab109115 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109115 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Selection and characterization of llama single domain antibodies against - Selection and characterization of llama single domain antibodies against

Schut MH, Pepers BA, Klooster R, van der Maarel SM, El Khatabi M, Verrips T, den Dunnen JT, van Ommen GJ, van Roon-Mom WM. Neurol Sci. 2015 Mar;36(3):429-34.