Anti-Huntingtin antibody - N-terminal
| Name | Anti-Huntingtin antibody - N-terminal |
|---|---|
| Supplier | Abcam |
| Catalog | ab83916 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to a N terminal region of human Huntingtin |
| Description | Rabbit Polyclonal |
| Gene | HTT |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Huntingtin antibody - N-terminal (ab83916) at 0.1 µg/mlLane 1 : Mouse brain extractLane 2 : Mouse brain extract with blocking peptide at 1 µg/ml
ICC/IF image of ab83916 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83916, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.