Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling HURP with ab70743 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-HURP antibody (ab70743) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µg
Detection of HURP by Western Blot of Immunprecipitate. ab70743 at 1µg/ml staining HURP in HeLa whole cell lysate immunoprecipitated using ab70743 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.