Anti-Hyaluronan synthase 1 antibody [3E10]
| Name | Anti-Hyaluronan synthase 1 antibody [3E10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab104864 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 3E10 |
| Applications | ELISA WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Purified recombinant fragment of Human Hyaluronan synthase 1 expressed in E |
| Description | Mouse Monoclonal |
| Gene | HAS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Hyaluronan synthase 1 antibody [3E10] (ab104864) at 1/500 dilution + Recombinant fragment (aa70-243) of Human Hyaluronan synthase 1](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5913_Hyaluronan-synthase-1-Primary-antibodies-ab104864-4.jpg)
Anti-Hyaluronan synthase 1 antibody [3E10] (ab104864) at 1/500 dilution + Recombinant fragment (aa70-243) of Human Hyaluronan synthase 1
Immunofluorescence analysis of U251 cells using ab104864 (green) at 1/200 dilution. Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
![Overlay histogram showing HCT116 cells stained with ab104864 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104864, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5915_Hyaluronan-synthase-1-Primary-antibodies-ab104864-5.jpg)
Overlay histogram showing HCT116 cells stained with ab104864 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104864, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Pirfenidone attenuates the IL-1beta-induced hyaluronic acid increase in orbital - Pirfenidone attenuates the IL-1beta-induced hyaluronic acid increase in orbital
Chung SA, Jeon BK, Choi YH, Back KO, Lee JB, Kook KH. Invest Ophthalmol Vis Sci. 2014 Apr 9;55(4):2276-83.
High-molecular-mass hyaluronan mediates the cancer resistance of the naked mole - High-molecular-mass hyaluronan mediates the cancer resistance of the naked mole
Tian X, Azpurua J, Hine C, Vaidya A, Myakishev-Rempel M, Ablaeva J, Mao Z, Nevo E, Gorbunova V, Seluanov A. Nature. 2013 Jul 18;499(7458):346-9.