Anti-Hyaluronan synthase 2 antibody [4E7]
| Name | Anti-Hyaluronan synthase 2 antibody [4E7] |
|---|---|
| Supplier | Abcam |
| Catalog | ab140671 |
| Prices | $394.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 4E7 |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Purified recombinant fragment, corresponding to amino acids 67-170 of Human Hyaluronan synthase 2, expressed in E |
| Description | Mouse Monoclonal |
| Gene | HAS2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab140671 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140671, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5918_ab140671-6-ab140671FC.jpg)
Overlay histogram showing HeLa cells stained with ab140671 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140671, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunofluorescenct analysis of HeLa cells labelling Hyaluronan synthase 2 with ab140671 at 1/100 dilution (green); Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Hyaluronan synthase 2 with ab140671 at 1/200 dilution followed by DAB staining.
![Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilution + Recombinant Human Hyaluronan synthase 2 protein](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5921_Hyaluronan-synthase-2-Primary-antibodies-ab140671-6.jpg)
Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilution + Recombinant Human Hyaluronan synthase 2 protein
![All lanes : Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilutionLane 1 : NTERA2 cell lysateLane 2 : HEK293 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5922_Hyaluronan-synthase-2-Primary-antibodies-ab140671-7.jpg)
All lanes : Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilutionLane 1 : NTERA2 cell lysateLane 2 : HEK293 cell lysate
Product References
Pirfenidone attenuates the IL-1beta-induced hyaluronic acid increase in orbital - Pirfenidone attenuates the IL-1beta-induced hyaluronic acid increase in orbital
Chung SA, Jeon BK, Choi YH, Back KO, Lee JB, Kook KH. Invest Ophthalmol Vis Sci. 2014 Apr 9;55(4):2276-83.
Calcipotriol counteracts betamethasone-induced decrease in extracellular matrix - Calcipotriol counteracts betamethasone-induced decrease in extracellular matrix
Norsgaard H, Kurdykowski S, Descargues P, Gonzalez T, Marstrand T, Dunstl G, Ropke M. Arch Dermatol Res. 2014 Oct;306(8):719-29.