Anti-Iba1 antibody

• Supplier: Abcam
• Catalog: ab5076
• Prices: $400.00
• Sizes: 100 µg
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F IHC-F IHC-P WB IHC-F ICC/IF ICC/IF ICC/IF
• Species Reactivities: Rat, Rabbit, Guinea Pig, Bovine, Human, Pig, Marmoset, Mouse, Monkey
• Antigen: Synthetic peptide: C-TGPPAKKAISELP , corresponding to amino acids 135-147 of Human Iba1(Peptide available as ab23067
• Description: Goat Polyclonal
• Gene: AIF1
• Conjugate: Unconjugated

Product images

Anti-Iba1 antibody (ab5076) at 2 µg/ml + Rat Brain lysate at 35 µg

Immunohistochemical analysis of paraffin embedded Mouse Brain tissue labeling Iba1 with ab5076 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.

IHC-P image of Iba1 staining on rat brain sections using ab5076 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab5076 was diluted 1:1000 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Rabbit polyclonal to anti sheep/goat IgG conjugated to biotin (1:200)See Abreview

IHC-P image of Iba1 staining on cat kidney sections using ab5076 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab5076 was diluted 1:1000 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Rabbit polyclonal to anti sheep/goat IgG conjugated to biotin (1:200)See Abreview

IHC-P image of Iba1 staining on cow kidney sections using ab5076 (1:2000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab5076 was diluted 1:2000 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Rabbit polyclonal to anti sheep/goat IgG conjugated to biotin (1:200)See Abreview

IHC-P image of Iba1 staining on marmoset bladder sections using ab5076 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab5076 was diluted 1:1000 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Rabbit polyclonal to anti goat IgG conjugated to biotin (1:200)See Abreview

ab5076 at 1/500 staining rat brain tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step was used, prior to incubation with the tissue for 30 minutes. An Alexa-Fluor ® 594 conjugated rabbit polyclonal antibody was used as the secondary.See Abreview

ab5076 staining Iba1 in guinea pig brain tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in sodium citrate and then blocking with 3% serum was performed for 30 minutes at RT. The primary antibody was used at dilution at 1/200 and incubated with sample at 2% blocking serum for 18 hours at 4°C. A Biotin conjugated horse polyclonal to goat IgG was used undiluted as secondary antibody.See Abreview

AIF1 expression in an inflammatory foci in a skin biopsy. Paraffin embedded sections were deparaffinized and boiled in 10mM citrate buffer for 30 min in a microwave to expose the AIF1 antigen, and then blocked with 5% donkey serum for 20 min. Slides were incubated for 40 min with goat-anti-AIF1 antibody (1:100) and rinsed in three changes of PBS for 1 min each.  A secondary antibody (donkey-anti-goat-FITC conjugated IgG; 1:50) was then applied to the slide for 40 min. Sections were washed again to remove the unbound antibody. The slides were counterstained with DaPI and viewed with a Nikon epi-fluorescent microscope. AIF1 positive cells appear green and the nuclei are stained blue. Numerous cells expressing the AIF1 protein are located around a vessel. (400X). Picture from Rreview by Carol Artlett submitted 30 July 2004.

    ab5076 at a 1/400 dilution staining rat brain tissue sections by Immunohistochemistry (frozen sections). The brain were frozen in isopentane, and the section postfixed with PFA2%. Following a short postfixation (15 minutes) the signal was very poor, while it improved after a longer fixation (1 hour). Heat mediated antigen retrieval was also attempted, but without a good result (excessive tissue specific aspecific binding, e.g. neurons), both on PFA postfixed frozen tissue and on tissue from perfused animals. An Alexa Fluor ® 568 conjugated antibody was used as the secondary antibody.This image is courtesy of an Abreview.See Abreview

Anti-Iba1 antibody (ab5076) at 0.5 µg/ml + Rat Brain lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

ab5076 staining Iba1 in rat spleen tissue by Immunohistochemistry (Frozen sections).Tissue was fixed with formaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab5076 at a 1/500 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated rabbit anti-goat polyclonal used at a 1/1000 dilution.See Abreview

ab5076 staining Iba1 in rat glioblastoma cell line C6 by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab5076 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a Cy3 conjugated rabbit anti-goat polyclonal used at a 1/120 dilution. Nuclei are counterstained with DAPI.See Abreview

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