AIF1

Gene Symbol AIF1
Entrez Gene 199
Alt Symbol AIF-1, IBA1, IRT-1, IRT1
Species Human
Gene Type protein-coding
Description allograft inflammatory factor 1
Other Description allograft inflammatory factor-1 splice variant Hara-1|interferon gamma responsive transcript|ionized calcium-binding adapter molecule 1|protein G1
Swissprots A8K406 Q9UKS9 P55008 Q9UIV4 O43904
Accessions AAD18087 BAB63392 CAA75060 CAA75061 CAA75062 EAX03442 EAX03443 EAX03444 EAX03445 EAX03446 EAX03447 P55008 AF088038 AF299327 AAG39110 AF299329 AAG39112 AK290771 BAF83460 BC009474 AAH09474 CB215777 CD521003 CR542153 CAG46950 D86438 BAA13088 DB502939 EF070981 EF070982 ABK35646 EF070983 ABK35647 EU832665 ACE87756 EU832744 ACE87076 JQ782414 AFL46387 JQ782415 AFL46388 U19713 AAB05003 U49392 AAA92457 U95213 AAC24422 XM_005248870 XP_005248927 XM_005248871 XP_005248928 XM_005272778 XP_005272835 XM_005272779 XP_005272836 XM_005274924 XP_005274981 XM_005274925 XP_005274982 XM_005275220 XP_005275277 XM_005275221 XP_005275278 XM_005275356 XP_005275413 XM_005275357 XP_005275414 XM_005275515 XP_005275572 XM_005275516 XP_005275573 NM_001623 NP_001614 NM_004847 NP_004838 NM_032955 NP_116573
Function Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. {ECO:0000269|PubMed:15117732, ECO:0000269|PubMed:16049345, ECO:0000269|PubMed:18699778}.
Subcellular Location Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
Tissue Specificity Detected in T-lymphocytes and peripheral blood mononuclear cells. {ECO:0000269|PubMed:16049345}.

Iba1 (1022-5) - sc-32725 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Iba1 (AIF-1): sc-32725. Immunoperoxidase staining of formalin fixed, paraffin-embedded rat C6 glioblastoma. Kindly provided by Dr. Hermann Schluesener at U. of Tuebingen Steinbeis Center, Germany.

Iba1 (F-4) - sc-398406 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Iba1 (F-4): sc-398406. Western blot analysis of Iba1 expression in human PBL whole cell lysate.

Iba1 (C-20) - sc-28530 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Iba1 (C-20): sc-28530. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.

Iba1 (N-20) - sc-28528 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Iba1 (N-20): sc-28528. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.

Iba1 (FL-147) - sc-98468 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Iba1 (FL-147): sc-98468. Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in white pulp and cytoplasmic and membrane staining of cells in red pulp.

Anti-Iba1 antibody - ab5076 from Abcam

Prices $400.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-Iba1 antibody (ab5076) at 2 µg/ml + Rat Brain lysate at 35 µg

Anti-Iba1 antibody - ab107159 from Abcam

Prices $392.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab107159 staining Iba1 in Rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted HRP-conjugated anti-goat IgG polyclonal was used as the secondary antibody. Kupffer cells are positive for Iba-1.See Abreview

Anti-Iba1 antibody - ab48004 from Abcam

Prices $385.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
ab48004 (2µg/ml) staining of paraffin embedded Mouse Brain shows staining of astrocytes. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-Iba1 antibody - ab108539 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Iba1 antibody (ab108539) at 1/500 dilution + Rat brain lysate

Anti-Iba1 antibody - ab153696 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Iba1 antibody (ab153696) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

Anti-Iba1 antibody - ab139590 from Abcam

Prices $376.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
IHC image of Iba1 staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab139590, 1µg/ml, for 15 mins at room temperature. A Goat anti-Chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Iba1 antibody - ab105464 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab105464 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab105464, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

Anti-Iba1 antibody - ab124355 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Iba1 antibody (ab124355) at 1/500 dilution + Human fetal brain lysate.

Anti-Iba1 antibody - ab96833 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Iba1 antibody (ab96833) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg

Anti-Iba1 antibody - ab166989 from Abcam

Host Sheep
Clonality Polyclonal

Anti-Iba1 antibody - ab54749 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Iba1 antibody [1022-5] - ab15690 from Abcam

Prices $391.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1022-5
ab15690 staining Iba1 in Rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with acetone and blocked with 5% BSA for 1 hour at 37°C. The sample was incubated with primary antibody (1/100) at 4°C for 18 hours. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.See Abreview

Anti-Iba1 antibody [1022-5] (FITC) - ab15691 from Abcam

Prices $387.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1022-5
Overlay histogram showing THP1 cells stained with ab15691 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15691, 10µl/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b (FITC) (ab91368, 10µl/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-Iba1 antibody [1022-5] (FITC) - ab110939 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1022-5
ab110939, at 1/50 dilution, staining Iba1 in formalin-fixed, paraffin-embedded Human Spleen tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Iba1 antibody [EPR16588] - ab178846 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16588
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Iba1 with ab178846 at a 1/2000 dilution showing cytoplasm and nuclear staining on Glial cells. Counter stained with hematoxylin. Prediluted HRP Polymer for Rabbit/Mouse IgG was used as the secondary aantibody. Negative control also shown. 

Anti-Iba1 antibody [EPR6136(2)] - ab178680 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6136(2)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Iba1 with ab178680 at 1/250 dilution.

Anti-AIF1 / IBA1 Antibody (clone 1022-5, Biotin) - LS-C96259 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1022-5

Anti-AIF1 / IBA1 Antibody - LS-C190045 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-AIF1 / IBA1 Antibody (clone GF1) - LS-C190046 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone GF1

Anti-AIF1 / IBA1 Antibody (aa13-44, APC) - LS-C251068 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Search more