Anti-Iba1 antibody

• Supplier: Abcam
• Catalog: ab107159
• Prices: $392.00
• Sizes: 100 µg
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F IHC-P WB
• Species Reactivities: Mouse, Rat, Dog, Human, Marmoset, Pig, Monkey, Hamster
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Rat Iba1
• Description: Goat Polyclonal
• Gene: AIF1
• Conjugate: Unconjugated

Product images

ab107159 staining Iba1 in Rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted HRP-conjugated anti-goat IgG polyclonal was used as the secondary antibody. Kupffer cells are positive for Iba-1.See Abreview

Anti-Iba1 antibody (ab107159) at 1 µg/ml + Rat Cortex Tissue Lysate at 10 µgSecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

IHC-P image of Iba1 staining on human brain sections using ab107159 (1:2000). The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The secondary antibody was horse polyclonal to anti-sheep conjugated to biotin at 1:200.See Abreview

IHC-P image of Iba1 staining on mouse spleen sections using ab107159 (1:2000). The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The secondary antibody was horse polyclonal to anti-sheep conjugated to biotin at 1:200.See Abreview

IHC-P image of Iba1 staining on rat spleen sections using ab107159 (1:2000). The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours. The secondary antibody was goat polyclonal to anti-sheep conjugated to biotin at 1:250.See Abreview

IHC-P image of Iba1 staining on marmoset (common) kidney sections using ab107159 (1:5000). The image shows macrophage infiltration in the cortex. The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 16 hours. The secondary antibody was rabbit polyclonal to sheep IgG conjugated to biotin at 1:200.See Abreview

Product References

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