ab48004 (2µg/ml) staining of paraffin embedded Mouse Brain shows staining of astrocytes. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Anti-Iba1 antibody (ab48004) at 0.5 µg/ml + Human frontal cortex lysate at 35 µgdeveloped using the ECL technique
All lanes : Anti-Iba1 antibody (ab48004) at 1 µg/mlLane 1 : Human Peripheral Blood Mononucleocyte lysate in RIPA bufferLane 2 : Human Peripheral Blood Mononucleocyte lysate in RIPA buffer with blocking peptideLysates/proteins at 35 µg per lane.developed using the ECL technique
ab48004 at 1/300 staining human jejunum tissue sections by IHC-P. A heat mediated antigen retrieval step in citric acid buffer was performed and the tissue was blocked in BSA before incubation with the antibody for 2 hours. A biotinylated rabbit anti-goat antibody was used as the secondary.See Abreview
ab48004 diluted 1/200 with TBS/BSA/Azide staining rat d15 embryonic liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat-mediated antigen retrieval step in citric acid buffer at pH6 was performed prior to blocking with 1% BSA for 10 minutes at room temperature. Incubation time with the primary antibody was 16 minutes. A biotinylated rabbit anti-goat was used as the secondarySee Abreview
ab48004 at 2.5ug/ml staining human placenta tissue sections by IHC-P. A heat mediated antigen retrieval step in citric acid buffer (pH 6) was performed.