Anti-Iba1 antibody (ab153696) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
All lanes : Anti-Iba1 antibody (ab153696) at 1/5000 dilutionLane 1 : THP1 whole cell lysateLane 2 : HL60 whole cell lysateLysates/proteins at 30 µg per lane.
Flow cytometic analysis of primary murine microglia cells labeling Iba1 with ab153696 at 1.0 µg per 4×105 cells (blue), Rabbit IgG (green) , Unstained (red).
Immunofluorescent analysis of HeLa cells (fixed in iced cold methanol; 5mins) labeling Iba1 with ab153696 at 1/500 dilution (left). Right image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human U373 xenograft tissue labeling Iba1 with ab153696 at 1/500 dilution.