Anti-Iba1 antibody [1022-5] (FITC)
| Name | Anti-Iba1 antibody [1022-5] (FITC) |
|---|---|
| Supplier | Abcam |
| Catalog | ab15691 |
| Prices | $387.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 1022-5 |
| Applications | FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | AIF1 |
| Conjugate | FITC |
| Supplier Page | Shop |
Product images
Overlay histogram showing THP1 cells stained with ab15691 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15691, 10µl/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b (FITC) (ab91368, 10µl/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
Effects of autoantigen and dexamethasone treatment on expression of - Effects of autoantigen and dexamethasone treatment on expression of
Schluesener HJ, Seid K, Meyermann R. Acta Neuropathol. 1999 Feb;97(2):119-26.
Allograft-inflammatory factor-1 in rat experimental autoimmune encephalomyelitis, - Allograft-inflammatory factor-1 in rat experimental autoimmune encephalomyelitis,
Schluesener HJ, Seid K, Kretzschmar J, Meyermann R. Glia. 1998 Oct;24(2):244-51.