Anti-Iba1 antibody [EPR16588]

• Supplier: Abcam
• Catalog: ab178846
• Prices: $376.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16588
• Applications: IHC-P WB
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Mouse Iba1 aa 100 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: AIF1
• Conjugate: Unconjugated

Product images

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Iba1 with ab178846 at a 1/2000 dilution showing cytoplasm and nuclear staining on Glial cells. Counter stained with hematoxylin. Prediluted HRP Polymer for Rabbit/Mouse IgG was used as the secondary aantibody. Negative control also shown. 

All lanes : Anti-Iba1 antibody [EPR16588] (ab178846) at 1/10000 dilutionLane 1 : THP-1 (Human monocytic leukemia cells) whole cell lysateLane 2 : U937 (Human histiocytic lymphoma cells) whole cell lysateLane 3 : Human spleen whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Anti-Iba1 antibody [EPR16588] (ab178846) at 1/2000 dilution + HL-60 (Human promyelocytic leukemia cells) whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Flow cytometry analysis of 2% paraformaldehyde fixed U937 (Human histiocytic lymphoma cells) cells labeling Iba1 with ab178846 at 1/160 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).