Anti-IDH2 antibody
| Name | Anti-IDH2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55271 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | IHC-P FC ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: HYREHQKGRP TSTNPIASIF AWTRGLEHRG KLDGNQDLIR FAQMLEKVCV ETVESGAMTK DLAGCIHGLS NVKLNEHFLN TTDFLDTIKS NLDRALGR, corresponding to amino acids 354-452 of Human IDH2 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | IDH2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IDH2 antibody (ab55271) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
Western blot against tagged recombinant protein immunogen using ab55271 IDH2 antibody at 1ug/ml.
ICC/IF image of ab55271 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55271, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-IDH2 antibody (ab55271)Lane 1 : IDH2 in transfected 293T cell line Lane 2 : Non-transfected lysate
![Overlay histogram showing MCF7 cells stained with ab55271 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55271, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/6327_IDH2-Primary-antibodies-ab55271-3.jpg)
Overlay histogram showing MCF7 cells stained with ab55271 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55271, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
MYC-driven accumulation of 2-hydroxyglutarate is associated with breast cancer - MYC-driven accumulation of 2-hydroxyglutarate is associated with breast cancer
Terunuma A, Putluri N, Mishra P, Mathe EA, Dorsey TH, Yi M, Wallace TA, Issaq HJ, Zhou M, Killian JK, Stevenson HS, Karoly ED, Chan K, Samanta S, Prieto D, Hsu TY, Kurley SJ, Putluri V, Sonavane R, Edelman DC, Wulff J, Starks AM, Yang Y, Kittles RA, Yfantis HG, Lee DH, Ioffe OB, Schiff R, Stephens RM, Meltzer PS, Veenstra TD, Westbrook TF, Sreekumar A, Ambs S. J Clin Invest. 2014 Jan;124(1):398-412.
D-2-hydroxyglutarate produced by mutant IDH2 causes cardiomyopathy and - D-2-hydroxyglutarate produced by mutant IDH2 causes cardiomyopathy and
Akbay EA, Moslehi J, Christensen CL, Saha S, Tchaicha JH, Ramkissoon SH, Stewart KM, Carretero J, Kikuchi E, Zhang H, Cohoon TJ, Murray S, Liu W, Uno K, Fisch S, Jones K, Gurumurthy S, Gliser C, Choe S, Keenan M, Son J, Stanley I, Losman JA, Padera R, Bronson RT, Asara JM, Abdel-Wahab O, Amrein PC, Fathi AT, Danial NN, Kimmelman AC, Kung AL, Ligon KL, Yen KE, Kaelin WG Jr, Bardeesy N, Wong KK. Genes Dev. 2014 Mar 1;28(5):479-90.
The potential for isocitrate dehydrogenase mutations to produce - The potential for isocitrate dehydrogenase mutations to produce
Ward PS, Lu C, Cross JR, Abdel-Wahab O, Levine RL, Schwartz GK, Thompson CB. J Biol Chem. 2013 Feb 8;288(6):3804-15.
Induction of sarcomas by mutant IDH2. - Induction of sarcomas by mutant IDH2.
Lu C, Venneti S, Akalin A, Fang F, Ward PS, Dematteo RG, Intlekofer AM, Chen C, Ye J, Hameed M, Nafa K, Agaram NP, Cross JR, Khanin R, Mason CE, Healey JH, Lowe SW, Schwartz GK, Melnick A, Thompson CB. Genes Dev. 2013 Sep 15;27(18):1986-98.
Cancer-associated IDH2 mutants drive an acute myeloid leukemia that is - Cancer-associated IDH2 mutants drive an acute myeloid leukemia that is
Chen C, Liu Y, Lu C, Cross JR, Morris JP 4th, Shroff AS, Ward PS, Bradner JE, Thompson C, Lowe SW. Genes Dev. 2013 Sep 15;27(18):1974-85.
IDH mutation impairs histone demethylation and results in a block to cell - IDH mutation impairs histone demethylation and results in a block to cell
Lu C, Ward PS, Kapoor GS, Rohle D, Turcan S, Abdel-Wahab O, Edwards CR, Khanin R, Figueroa ME, Melnick A, Wellen KE, O'Rourke DM, Berger SL, Chan TA, Levine RL, Mellinghoff IK, Thompson CB. Nature. 2012 Feb 15;483(7390):474-8.
Isocitrate dehydrogenase (IDH) mutations promote a reversible ZEB1/microRNA - Isocitrate dehydrogenase (IDH) mutations promote a reversible ZEB1/microRNA
Grassian AR, Lin F, Barrett R, Liu Y, Jiang W, Korpal M, Astley H, Gitterman D, Henley T, Howes R, Levell J, Korn JM, Pagliarini R. J Biol Chem. 2012 Dec 7;287(50):42180-94.
Microarray and proteomic analysis of the cardioprotective effects of cold blood - Microarray and proteomic analysis of the cardioprotective effects of cold blood
Black KM, Barnett RJ, Bhasin MK, Daly C, Dillon ST, Libermann TA, Levitsky S, McCully JD. Physiol Genomics. 2012 Nov 1;44(21):1027-41. doi:
Hypoxia promotes isocitrate dehydrogenase-dependent carboxylation of - Hypoxia promotes isocitrate dehydrogenase-dependent carboxylation of
Wise DR, Ward PS, Shay JE, Cross JR, Gruber JJ, Sachdeva UM, Platt JM, DeMatteo RG, Simon MC, Thompson CB. Proc Natl Acad Sci U S A. 2011 Dec 6;108(49):19611-6. doi:
The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic - The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic
Ward PS, Patel J, Wise DR, Abdel-Wahab O, Bennett BD, Coller HA, Cross JR, Fantin VR, Hedvat CV, Perl AE, Rabinowitz JD, Carroll M, Su SM, Sharp KA, Levine RL, Thompson CB. Cancer Cell. 2010 Mar 16;17(3):225-34.