Anti-IDH2 antibody [EPR7576]

• Supplier: Abcam
• Catalog: ab129180
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR7576
• Applications: WB IHC-P FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues in Human IDH2
• Description: Rabbit Monoclonal
• Gene: IDH2
• Conjugate: Unconjugated

Product images

Overlay histogram showing MCF7 cells stained with ab129180 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129180, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

ab129180, at 1/250 dilution, staining IDH2 in Formalin-fixed, Paraffin-embedded Human thyroid gland carcinoma by Immunohistochemistry.

All lanes : Anti-IDH2 antibody [EPR7576] (ab129180) at 1/1000 dilutionLane 1 : Molt-4 cell lysateLane 2 : K562 cell lysateLane 3 : 293T cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-rabbit HRP