Flow cytometric analysis of A549 cells (green) compared to a negative control cell (blue) using ab170453 at a 1/10 dilution. FITC conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of A549 cells using ab170453 at a 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling IEX1 with ab170453 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Anti-IEX1 antibody - N-terminal (ab170453) at 1/100 dilution + A549 cell line lysate at 35 µg
Anti-IEX1 antibody - N-terminal (ab170453) at 1/100 dilution + Mouse heart tissue lysate at 35 µg