Anti-IGFBP3 antibody
| Name | Anti-IGFBP3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab76001 |
| Prices | $385.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA IHC-P |
| Species Reactivities | Human |
| Antigen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S183 of Human IGFBP3 |
| Description | Rabbit Polyclonal |
| Gene | IGFBP3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-IGFBP3 antibody (ab76001) at 1/50 dilutionLane 1 : Lysates from non-transfected 293 cells Lane 2 : Lysates form 293 cells transiently transfected with IGFBP3 gene Lysates/proteins at 2 µg per lane.
ab76001 at 1/50 dilution, staining IGFBP3 in human breast carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). The DAB staining procedure was used for detection.
ICC/IF image of ab76001 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76001, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.