All lanes : Anti-Ikaros [EPR13790] antibody (ab191394) at 1/10000 dilutionLane 1 : Raji cell lysateLane 2 : Ramos cell lysateLane 3 : MOLT4 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling Ikaros with ab191394 at 1/1400 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.Inset: Negative control: using PBS instead of primary antibody.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Ikaros with ab191394 at 1/1400 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.Inset: Negative control: using PBS instead of primary antibody.
Flow Cytometric analysis of MOLT4 cells (paraformaldehyde-fixed, 2%) labeling Ikaros with ab191394 at 1/130 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.