Anti-IKK alpha + IKK beta [EPR16628] antibody

• Supplier: Abcam
• Catalog: ab178870
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16628
• Applications: IP ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human IKK alpha + IKK beta aa 450 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: CHUK
• Conjugate: Unconjugated

Product images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling IKK alpha + IKK beta with ab178870 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody (ab150077) at 1/200 dilution (green). Nuclear and cytoplasm staining is detected. Tubulin is detected with Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). The nuclear counter stain is DAPI (blue).  The two negative controls were :--ve control 1 - ab178870 at 1/50 diltuion followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.-ve control 2 - Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution.

All lanes : Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 2 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysatesLane 3 : HL-60 (Human promyelocytic leukemia cells) whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

All lanes : Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilutionLane 1 : A431 (Human epidermoid carcinoma) whole cell lysatesLane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution + Human fetal kidney lysates at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

All lanes : Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilutionLane 1 : Mouse brain lysatesLane 2 : Mouse kidney lysatesLane 3 : Mouse spleen lysatesLane 4 : Rat kidney lysatesLane 5 : C6 (Rat glial tumor cells) whole cell lysatesLane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysatesLane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using ab178870 at 1/40 dilution. Western blot detection was performed using ab178870 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST. ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.  

Cross-Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using ab178870 at 1/40 dilution. Western blot detection was performed using ab32041 (IKK alpha) at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST.

This immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells is a comparison between ab178870 and a competitor’s leading rabbit polyclonal antibody.Labeling of IKK alpha + IKK beta with ab178870 is conducted at 1/50 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) secondary antibody (ab150077) at 1/200 dilution.Labeling of IKK alpha + IKK beta with the competitor antibody is conducted at 1/40 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor ® 488) at 1/200 dilution.Tubulin is detected with Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). The nuclear counter stain is DAPI (blue).The two negative controls were :-ve control 1 - ab178870 at 1/50 diltuion followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.-ve control 2 - Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution f

All lanes : Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 2 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysatesLane 3 : HL-60 (Human promyelocytic leukemia cells) whole cell lysatesSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

All lanes : Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysatesLane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysatesSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200 dilution + Human fetal kidney lysatesSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

All lanes : Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200 dilutionLane 1 : Mouse brain lysatesLane 2 : Mouse kidney lysatesLane 3 : Mouse spleen lysatesLane 4 : Rat kidney lysatesLane 5 : C6 (Rat glial tumor cells) whole cell lysatesLane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysatesLane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysatesSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

This immunoprecipation image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using ab178870 at 1/40 dilution. Western blot detection was performed using ab178870 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST.Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using competitor’s Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/12 dilution. Western blot detection was performed using competitor antibody at 1/100 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/100 dilution. The blocking and diluting buffer was 5% NFDM/TBST. ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively.