Anti-IKKi/IKKe antibody
| Name | Anti-IKKi/IKKe antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7891 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P IP WB |
| Species Reactivities | Human |
| Antigen | Peptide ( NRIIERLNRVPAPPDV ) corresponding to aminoacids 701 to 716 of human IKKe/IKK-i (1,2) |
| Description | Rabbit Polyclonal |
| Gene | IKBKE |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml + Jurkat whole cell lysate
ab7891 at 10µg/ml staining IKK epsilon in human pancreatic tissue by IHC
ICC/IF image of ab7891 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7891, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Human DEAD box helicase 3 couples IkappaB kinase epsilon to interferon regulatory - Human DEAD box helicase 3 couples IkappaB kinase epsilon to interferon regulatory
Gu L, Fullam A, Brennan R, Schroder M. Mol Cell Biol. 2013 May;33(10):2004-15.
Cellular localization of the herpes simplex virus ICP0 protein dictates its - Cellular localization of the herpes simplex virus ICP0 protein dictates its
Paladino P, Collins SE, Mossman KL. PLoS One. 2010 Apr 29;5(4):e10428.
Peripheral B cells may serve as a reservoir for persistent hepatitis C virus - Peripheral B cells may serve as a reservoir for persistent hepatitis C virus
Ito M, Masumi A, Mochida K, Kukihara H, Moriishi K, Matsuura Y, Yamaguchi K, Mizuochi T. J Innate Immun. 2010;2(6):607-17.
Viral targeting of DEAD box protein 3 reveals its role in - Viral targeting of DEAD box protein 3 reveals its role in
Schroder M, Baran M, Bowie AG. EMBO J. 2008 Aug 6;27(15):2147-57.
IkappaB kinase epsilon interacts with p52 and promotes transactivation via p65. - IkappaB kinase epsilon interacts with p52 and promotes transactivation via p65.
Wietek C, Cleaver CS, Ludbrook V, Wilde J, White J, Bell DJ, Lee M, Dickson M, Ray KP, O'Neill LA. J Biol Chem. 2006 Nov 17;281(46):34973-81. Epub 2006 Sep 26.