IKBKE

Gene Symbol IKBKE
Entrez Gene 9641
Alt Symbol IKK-E, IKK-i, IKKE, IKKI
Species Human
Gene Type protein-coding
Description inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
Other Description I-kappa-B kinase epsilon|IKK-epsilon|IKK-related kinase epsilon|inducible I kappa-B kinase|inducible IkappaB kinase|inhibitor of nuclear factor kappa-B kinase subunit epsilon
Swissprots Q14164 Q5JTS6 D3DT78 Q3KR43 Q3B754
Accessions ABG25921 CAC09147 CAI15250 CAT02203 EAW93547 EAW93548 EAW93549 Q14164 AB016590 BAA85155 AF241789 AAF45307 AK093798 AK310737 AL706097 AY660028 BC105923 AAI05924 BC105924 AAI05925 BC107591 BC107812 AAI07813 CB243308 D63485 BAA09772 DA990116 DA999897 DB152295 XM_005273356 XP_005273413 XR_922002 NM_001193321 NP_001180250 NM_001193322 NP_001180251 NM_014002 NP_054721
Function Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG- I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1,
Subcellular Location Cytoplasm. Nucleus. Nucleus, PML body. Note=Targeting to PML nuclear bodies upon DNA damage is TOPORS- dependent.
Tissue Specificity Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
Top Pathways Toll-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Cytosolic DNA-sensing pathway, Hepatitis C, Measles

Phospho-IKKε (Ser172) (D1B7) Rabbit mAb - 8766 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1B7
Western blot analysis of extracts from THP-1 cells, differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml, indicated times), using Phospho-IKKε (Ser172) (D1B7) Rabbit mAb (upper) or IKKε (D20G4) Rabbit mAb #2905 (lower).

IKKε (D61F9) XP ® Rabbit mAb - 3416 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D61F9
Western blot analysis of extracts from Raw 264.7 and KNRK cell lines using IKKε (D61F9) XP ® Rabbit mAb.

IKKε Antibody - 2690 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of recombinant IKKε Kinase #7553 and RAW 264.7 cells, untreated or LPS-treated, using IKKε Antibody.

IKKε (D20G4) Rabbit mAb - 2905 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D20G4
Western blot analysis of recombinant GST-IKKε #7553 and lysates from Ramos and RL7 cells using IKKε (D20G4) Rabbit mAb.

IKKε Antibody - 2686 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

IKK-i (A-11) - sc-376114 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IKK-i (A-11): sc-376114. Western blot analysis of IKK-i expression in LPS treated RAW 309 Cr.1 whole cell lysate.

IKK-i (C-9) - sc-365830 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IKK-i (C-9): sc-365830. Western blot analysis of IKK-i expression in LB treated RAW 309 Cr.1 whole cell lysate.

IKK-i (6B4B5) - sc-101430 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IKK-i (6B4B5): sc-101430. Western blot analysis of IKK-i expression in non-transfected: sc-117752 (A) and mouse IKK-i transfected: sc-121028 (B) 293T whole cell lysates.

IKK-i (H-116) - sc-10760 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
IKK-i (H-116): sc-10760. Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.

IKK-i (K-14) - sc-5694 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
IKK-i (K-14): sc-5694. Western blot analysis of IKK-i expression in RAW 309 Cr.1 whole cell lysate.

IKK-i (Q-15) - sc-9913 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
IKK-i (Q-15): sc-9913. Immunofluorescence staining of methanol-fixed RAW 309 Cr.1 cells showing localizedcytoplasmic staining.

IKK-i (72B587) - sc-52931 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
IKK-i (72B587): sc-52931. Western blot analysis of IKK-i expression in Jurkat (A), RAW 309 Cr.1 (B), MCF7 (C), Daudi (D) and HeLa (E) whole cell lysates and mouse liver tissue extract (F).

Anti-IKKi/IKKe antibody - ab7891 from Abcam

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml + Jurkat whole cell lysate

Anti-IKKi/IKKe antibody - ab12143 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-IKKi/IKKe antibody [72B587] - ab12142 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 72B587
ICC/IF image of ab12142 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12142, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-IKKi/IKKe antibody [EPR4904] - ab124766 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4904
All lanes : Anti-IKKi/IKKe antibody [EPR4904] (ab124766) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : Raji cell lysateLane 3 : MCF7 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-IKKi/IKKe antibody - Aminoterminal end - ab70847 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-IKKi/IKKe antibody - Aminoterminal end (ab70847) at 1/100 dilution + THP-1 cell lysate. at 12.5 µg

Anti-IKKi/IKKe antibody - C-terminal - ab155794 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-IKKi/IKKe antibody - C-terminal (ab155794) at 1/1000 dilution + 293T whole cell lysate at 30 µg

Anti-IKKi/IKKe antibody - Carboxyterminal end - ab37596 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-IKKi/IKKe antibody - Carboxyterminal end (ab37596) at 1/100 dilution + HEK293 cell lysate

Anti-IKBKE / IKKI / IKKE Antibody (aa175-188) - LS-C15936 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-IKBKE / IKKI / IKKE Antibody (phospho-Thr501) - LS-C15937 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-IKBKE / IKKI / IKKE Antibody (C-Terminus) - LS-C70504 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-IKBKE / IKKI / IKKE Antibody (aa175-188) - LS-C70509 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-IKBKE / IKKI / IKKE Antibody (clone 1F7) - LS-C105086 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1F7

Anti-IKBKE / IKKI / IKKE Antibody (aa1-257) - LS-C41522 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Western blot using IKBKE mouse monoclonal antibody against truncated IKBKE recombinant protein (1) and full-length IKBKE(aa1-716)-hIgGFc transfected COS7 cell lysate (2).
Search more