![IKZF3 was immunoprecipitated using 0.5mg Raji whole cell extract, 5µg of Rabbit polyclonal to IKZF3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Raji whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64400.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 64kDa; IKZF3](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/7298_ab64400-195128-IPV021ab6440020minM.jpg)
IKZF3 was immunoprecipitated using 0.5mg Raji whole cell extract, 5µg of Rabbit polyclonal to IKZF3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Raji whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64400.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 64kDa; IKZF3
All lanes : Anti-IKZF3 antibody (ab64400) at 1 µg/mlLane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.