IKZF3

Gene Symbol	IKZF3
Entrez Gene	22806
Alt Symbol	AIO, AIOLOS, ZNFN1A3
Species	Human
Gene Type	protein-coding
Description	IKAROS family zinc finger 3 (Aiolos)
Other Description	zinc finger DNA binding protein Aiolos\|zinc finger protein Aiolos\|zinc finger protein, subfamily 1A, 3 (Aiolos)
Swissprots	Q69BM1 Q8WWR1 Q8WWQ9 Q69BM5 B4DVV5 Q69BL8 Q69BL7 Q69BL6 Q69BL9 Q69BM0 Q8N574 Q8WWR0 Q69BM2 Q9UKT9 Q8WWR2 Q69BM3 Q8WWR3
Accessions	EAW60604 EAW60605 EAW60606 EAW60607 EAW60608 EAW60609 Q9UKT9 AF086054 AF129512 AAF13493 AI866838 AJ292565 CAC80427 AJ292566 CAC80428 AJ292567 CAC80429 AJ292568 CAC80430 AJ292569 CAC80431 AK128436 AK301250 BAG62817 AK303495 BAG64529 AY377973 AAR84584 AY377975 AAR84586 AY377976 AAR84587 AY377977 AAR84588 AY377978 AAR84589 AY377979 AAR84590 AY377980 AAR84591 AY377981 AAR84592 AY377982 AAR84593 BC032707 AAH32707 EU446776 ABZ92305 HG509027 JF432186 ADZ15403 NM_001257408 NP_001244337 NM_001257409 NP_001244338 NM_001257410 NP_001244339 NM_001257411 NP_001244340 NM_001257412 NP_001244341 NM_001257413 NP_001244342 NM_001257414 NP_001244343 NM_001284514 NP_001271443 NM_001284515 NP_001271444 NM_001284516 NP_001271445 NM_012481 NP_036613 NM_183228 NP_899051 NM_183229 NP_899052 NM_183230 NP_899053 NM_183231 NP_899054 NM_183232 NP_899055
Function	Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2- dependent manner. {ECO:0000269\|PubMed:10369681}.
Subcellular Location	Nucleus. Cytoplasm.
Tissue Specificity	Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. {ECO:0000269\|PubMed:17646674}.

Aiolos Antibody - 12720 from Cell Signaling Technology

Prices	$239.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human Aiolos (hAiolos; +), using Aiolos Antibody.

Aiolos (O-21) - sc-101982 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Rabbit
Clonality	Polyclonal

Aiolos (O-21): sc-101982. Western blot analysis of Aiolos expression in Hep G2 whole cell lysate.

Aiolos (Y-13) - sc-160013 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Aiolos (Y-13): sc-160013. Western blot analysis of Aiolos expression in non-transfected: sc-117750 (A), human Aiolos transfected: sc-115233 (B) and Raji (C) whole cell lysates.

Anti-IKZF3 antibody - ab69930 from Abcam

Prices	$379.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-IKZF3 antibody (ab69930) at 1/500 dilutionLane 1 : COLO cell extractLane 2 : COLO cell extract with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-IKZF3 antibody - ab199111 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling IKZF3 using ab199111 at a 1/60 dilution.

Anti-IKZF3 antibody - ab88513 from Abcam

Prices	$376.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-IKZF3 antibody (ab88513) at 1 µg/ml + Jurkat cell lysate at 50 µg

Anti-IKZF3 antibody - ab64400 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IKZF3 was immunoprecipitated using 0.5mg Raji whole cell extract, 5µg of Rabbit polyclonal to IKZF3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Raji whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64400.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 64kDa; IKZF3

Anti-IKZF3 antibody - ab174879 from Abcam

Prices	$376.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IKZF3 antibody (ab174879) at 1/100 dilution + Mouse liver tissue lysates at 15 µg

Anti-IKZF3 antibody - ab134288 from Abcam

Prices	$376.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with ab134288 at 10 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-IKZF3 antibody - ab56867 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Western blot against tagged recombinant protein immunogen using ab56867 IKZF3 antibody at 1ug/ml.

Anti-IKZF3 antibody [EPR9342(B)] - ab139408 from Abcam

Prices	$381.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR9342(B)

All lanes : Anti-IKZF3 antibody [EPR9342(B)] (ab139408) at 1/20000 dilution (purified)Lane 1 : Raji cell lysateLane 2 : Ramos cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-IKZF3 antibody [EPR9343(B)] - ab139182 from Abcam

Prices	$379.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR9343(B)

All lanes : Anti-IKZF3 antibody [EPR9343(B)] (ab139182) at 1/1000 dilutionLane 1 : Raji cell lysateLane 2 : Ramos cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-IKZF3 / AIOLOS Antibody (C-Terminus) - LS-C80247 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-IKZF3 / AIOLOS Antibody (aa1-510, clone 10B2563, Azide-free) - LS-C183727 from LifeSpan Bioscience

Prices	$485.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	10B2563

Anti-IKZF3 / AIOLOS Antibody (aa1-510, PE) - LS-C246468 from LifeSpan Bioscience

Prices	$620.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal

Anti-IKZF3 / AIOLOS Antibody (aa76-103, AP) - LS-C246772 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IKZF3 / AIOLOS Antibody (aa76-103, APC) - LS-C246773 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IKZF3 / AIOLOS Antibody (aa76-103, Biotin) - LS-C246774 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IKZF3 / AIOLOS Antibody (aa76-103, FITC) - LS-C246775 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IKZF3 / AIOLOS Antibody (aa361-410) - LS-C119318 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of extracts from COLO cells, using IKZF3 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-IKZF3 / AIOLOS Antibody (aa76-103, PE) - LS-C246777 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IKZF3 / AIOLOS Antibody (aa76-103, Azide-free, HRP) - LS-C249259 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IKZF3 / AIOLOS Antibody - LS-C308437 from LifeSpan Bioscience

Prices	$455.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-IKZF3 / AIOLOS Antibody (aa83-112) - LS-C322195 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IKZF3 / AIOLOS Antibody (aa1-301, clone 1E1) - LS-C339096 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1E1

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IKZF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IKZF3.

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