Anti-IL1 alpha antibody
| Name | Anti-IL1 alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab9614 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | unknown |
| Applications | ICC/IF ICC/IF ELISA B/N WB IHC-P |
| Species Reactivities | Human |
| Antigen | Highly pure (>98%) recombinant hIL-1a (human Interleukin-1-alpha) |
| Description | Rabbit Polyclonal |
| Gene | IL1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab9614 at 1.0 µg/ml staining normal human skin by IHC-P. The antibody was incubated overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen. Enzyme induced antigen retrieval was performed with proteinase K.
ab9614 at 1/50 staining IL1 in human primary tonsillar epithelial cell by immunocytochemistry/ immunofluorescence. The cells were paraformaldehyde fixed and blocked with 20% serum prior to incubation with the antibody for 1 hour. Alexa fluor® 594 goat polyclonal to rabbit Ig, diluted 1/1000, was used as the secondary antibody.See Abreview
Anti-IL1 alpha antibody (ab9614) at 1 µg/ml + Active human IL1 alpha full length protein (ab73567) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds
Product References
The NLRP3/ASC inflammasome promotes T-cell-dependent immune complex - The NLRP3/ASC inflammasome promotes T-cell-dependent immune complex
Andersen K, Eltrich N, Lichtnekert J, Anders HJ, Vielhauer V. Kidney Int. 2014 Nov;86(5):965-78.
IL-1alpha/IL-1R1 expression in chronic obstructive pulmonary disease and - IL-1alpha/IL-1R1 expression in chronic obstructive pulmonary disease and
Botelho FM, Bauer CM, Finch D, Nikota JK, Zavitz CC, Kelly A, Lambert KN, Piper S, Foster ML, Goldring JJ, Wedzicha JA, Bassett J, Bramson J, Iwakura Y, Sleeman M, Kolbeck R, Coyle AJ, Humbles AA, Stampfli MR. PLoS One. 2011;6(12):e28457.