Anti-IL11RA antibody [EPR5446]

• Supplier: Abcam
• Catalog: ab125015
• Prices: $313.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5446
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: A synthetic peptide, corresponding to residues in Human IL11RA
• Description: Rabbit Monoclonal
• Gene: IL11RA
• Conjugate: Unconjugated

Product images

Overlay histogram showing K562 cells stained with ab125015 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125015, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-IL11RA antibody [EPR5446] (ab125015) at 1/1000 dilutionLane 1 : 293T lysateLane 2 : Saos2 lysateLane 3 : A673 lysateLane 4 : PC3 lysateLane 5 : Hut78 lysateLane 6 : K562 lysateLane 7 : TF1 lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

ab125015 at 1/500 staining IL11RA in paraffin embedded Human Kidney tissue by immunohistochemistry.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD