Anti-IL1RA antibody
| Name | Anti-IL1RA antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2573 |
| Prices | $397.00 |
| Sizes | 500 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA FC IHC-P IHC-F B/N RIA WB |
| Species Reactivities | Human, Primate |
| Antigen | Recombinant human IL-1Ra produced in E |
| Description | Rabbit Polyclonal |
| Gene | IL1RN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab2573 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2573, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-IL1RA antibody (ab2573) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Anti-IL1RA antibody (ab2573) at 1 µg/ml + Human IL1RA full length protein (ab50081) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 20 seconds
Product References
Mutant p53 gains new function in promoting inflammatory signals by repression of - Mutant p53 gains new function in promoting inflammatory signals by repression of
Ubertini V, Norelli G, D'Arcangelo D, Gurtner A, Cesareo E, Baldari S, Gentileschi MP, Piaggio G, Nistico P, Soddu S, Facchiano A, Bossi G. Oncogene. 2014 Jul 7.
Interleukin 1alpha sustains the expression of inflammatory factors in human - Interleukin 1alpha sustains the expression of inflammatory factors in human
Tjomsland V, Spangeus A, Valila J, Sandstrom P, Borch K, Druid H, Falkmer S, Falkmer U, Messmer D, Larsson M. Neoplasia. 2011 Aug;13(8):664-75.