All lanes : Anti-IL1RA antibody (ab135570) at 1/50 dilutionLane 1 : Non transfected 293 cell lysate Lane 2 : 293 cell lysate transiently transfected with the IL1RA geneLysates/proteins at 2 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labelled with ab135570 at 1/50 dilution. ab135570 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of 293 cells, using ab135570 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of ab135570 at 1/10 dilution with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).