Anti-IL1RA antibody [EPR6483]

• Supplier: Abcam
• Catalog: ab124962
• Prices: $394.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR6483
• Applications: WB IP IHC-P FC ICC/IF ICC/IF IHC-F ELISA
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues at the C-terminal of Human IL1RA antagonist
• Description: Rabbit Monoclonal
• Gene: IL1RN
• Conjugate: Unconjugated

Product images

Overlay histogram showing A431 cells stained with ab124962 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124962, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-IL1RA antibody [EPR6483] (ab124962) at 1/10000 dilutionLane 1 : A431 cell lysatesLane 2 : 293T cell lysatesLane 3 : MOLT4 cell lysatesLane 4 : HeLa cell lysatesLane 5 : RAW264.7 cell lysatesLane 6 : NIH 3T3 cell lysatesLane 7 : L6 cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

ab124962, at a 1/100 dilution, staining IL1RA in paraffin embedded Human kidney tissue by Immunohistochemistry.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD