Anti-IL20 antibody [15]
| Name | Anti-IL20 antibody [15] |
|---|---|
| Supplier | Abcam |
| Catalog | ab51708 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 15 |
| Applications | ICC/IF ICC/IF ELISA WB IHC-F IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide located in interleukin 20 short form (IL-20s) |
| Description | Mouse Monoclonal |
| Gene | IL20 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-IL20 antibody [15] (ab51708) at 1/1000 dilutionLane 1 : Human skin whole tissue lysateLane 2 : Human skin whole tissue lysateLane 3 : Human skin whole tissue lysateLysates/proteins at 30 µg per lane.SecondaryHRP-conjugated Horse anti-mouse IgG polyclonal at 1/2000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/7778_ab51708-210086-ab51708wb.jpg)
All lanes : Anti-IL20 antibody [15] (ab51708) at 1/1000 dilutionLane 1 : Human skin whole tissue lysateLane 2 : Human skin whole tissue lysateLane 3 : Human skin whole tissue lysateLysates/proteins at 30 µg per lane.SecondaryHRP-conjugated Horse anti-mouse IgG polyclonal at 1/2000 dilutionPerformed under reducing conditions.
ICC/IF image of ab51708 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51708, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.