Anti-IL6 antibody

• Supplier: Abcam
• Catalog: ab6672
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ELISA IHC-P RIA IP IHC-F B/N ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human, Pig
• Antigen: recombinant human IL-6 produced in E
• Description: Rabbit Polyclonal
• Gene: IL6
• Conjugate: Unconjugated

Product images

ab6672 staining IL6 (red)  in Mouse mesenchimal SC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Tween-20 and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in 3% BSA) for 1 hour. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1:500) was used as the secondary antibody. Blue (DAPI) - nuclei.See Abreview

Anti-IL6 antibody (ab6672) at 1/500 dilution + Endotoxin-stimulated human peripheral blood mononuclear cells

The samples are formalin-fixed, formic acid decalcified rat knee joints with type II collagen induced arthritis. This image was kindly supplied as part of the review submitted by Elizabeth Chlipala (at University of Colorado, USA).

This picture represents a tissue section from the wrist joint in collagen-induced arthritis in mice stained with ab6672.  This image was kindly supplied as part of the review submitted by Elizabeth Chlipala.

Anti-IL6 antibody (ab6672) at 1/1000 dilutionFigure. Immunoblot using Abcam's anti- IL6 antibody. This blot shows detection of an IL-6 26kDa tagged fusion protein (~300 ng, lane 1, green, 800 nm channel). Minimal reactivity is observed against the tag (data not shown). Protein was resolved on a 4-20% Tris- Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of a fluorescent conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature. Molecular weight markers are shown for size comparison (red, 700 nm channel). Other detection systems will yield similar results.

ab6672 staining IL6 in human WBC by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with acetone and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in PBS; 0.1% TX10; 1% Goat serum) for 16 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody. See Abreview

ab6672 staining IL6 in Mouse pancreatic neoplastic lesion tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with PBS + Triton (0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated Goat anti-mrabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained with DAPI.See Abreview

ab6672 staining IL6 in serum starved PC3 cells treated with TRAP-6 (ab120801), by ICC/IF. Increase in expression of IL6 correlates with increased concentration of TRAP-6, as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ab120801 (TRAP-6) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6672 (1/500 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

All lanes : Anti-IL6 antibody (ab6672) at 1/1000 dilutionLane 1 : Mouse serumLane 2 : Mouse serumLysates/proteins at 0.3 µg per lane.SecondaryIRDye® 800CW goat anti-rabbit IgG (H+L) polyclonal at 1/15000 dilutionPerformed under reducing conditions.Observed band size : 17 kDa (why is the actual band size different from the predicted?)Exposure time : 5 minutesThis image is courtesy of an Abreview submitted by Francesco Elia MarinoSee Abreview

Product References

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