All lanes : Anti-IL6R antibody [B-N12] (ab27404) at 5 µg/mlLane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionObserved band size : 53 kDa (why is the actual band size different from the predicted?)Additional bands at : 40 kDa (possible isoform).