Anti-Importin4 antibody
| Name | Anti-Importin4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28387 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Importin4 |
| Description | Rabbit Polyclonal |
| Gene | IPO4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Importin4 antibody (ab28387) at 1 µg/mlLane 1 : Hela B Cell Lysate at 30 µgLane 2 : Hela B Cell Lysate at 60 µg
All lanes : Anti-Importin4 antibody (ab28387) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate - TPA stimulated 2 hr (ab14653)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab28387 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28387, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab28387 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28387, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Beta-like importins mediate the nuclear translocation of mitogen-activated - Beta-like importins mediate the nuclear translocation of mitogen-activated
Zehorai E, Seger R. Mol Cell Biol. 2014 Jan;34(2):259-70.
Sequential establishment of marks on soluble histones H3 and H4. - Sequential establishment of marks on soluble histones H3 and H4.
Alvarez F, Munoz F, Schilcher P, Imhof A, Almouzni G, Loyola A. J Biol Chem. 2011 May 20;286(20):17714-21.