Anti-INCENP antibody [58-217]
| Name | Anti-INCENP antibody [58-217] |
|---|---|
| Supplier | Abcam |
| Catalog | ab23956 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 58-217 |
| Applications | ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | His-tagged human INCENP fragment aa231-298 |
| Description | Mouse Monoclonal |
| Gene | INCENP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab23956 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23956, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab23956 staining INCENP in HeLa cells, by immunofluorescence. optimal antibody dilution: 1mg/ml optimal fixation protocol: PFA/Triton fixation: 10 min room temperature in 3,7 % PFA diluted in PHEM PEM buffer: 45 mM Hepes pH 6,9, 45 mM Pipes pH 6,9, 5mM MgCl2, 10 mM EGTA) containing 0 ,2% Triton X-100, followed by 3 washes in PBS. Also gives a good staining with PFA fixation: 10 min in 3,7 % PFA at room temperature, 5 min in PBS / 0,1%Triton X-100, then 3 washes in PBS.IF staining was performed following a standard protocol: blocking: 30 min; primary antibody: 1 hr; secondary antibody: 45’. All incubations were at 37 °C in PBS/ 0,1% Tween containing 3% BSA.This antibody was also tested using NIH-3T3 cells but it did not provide good results with these cells.
ab23956 staining human anal cancer tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in citrate buffer (pH 6) prior to blocking in a commercially available blocking agent for 20 minutes at 20°C. The primary anibody was diluted 1/200 and incubated with the sample for 40 minutes at 20°C. A HRP-conjugated goat anti-mouse antibody was used as the secondary.See Abreview
ab23956 staining INCENP in siRNA-treated HeLa cells by Immunocytochemistry/ Immunofluorescence.
Product References
Functional interplay between Aurora B kinase and Ssu72 phosphatase regulates - Functional interplay between Aurora B kinase and Ssu72 phosphatase regulates
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Raptor, a positive regulatory subunit of mTOR complex 1, is a novel - Raptor, a positive regulatory subunit of mTOR complex 1, is a novel
Vazquez-Martin A, Cufi S, Oliveras-Ferraros C, Menendez JA. Cell Cycle. 2011 Sep 15;10(18):3140-52. Epub 2011 Sep 15.
Defects in nuclear pore assembly lead to activation of an Aurora B-mediated - Defects in nuclear pore assembly lead to activation of an Aurora B-mediated
Mackay DR, Makise M, Ullman KS. J Cell Biol. 2010 Nov 29;191(5):923-31.