Anti-ING2 antibody [EPR4597]

• Supplier: Abcam
• Catalog: ab109504
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4597
• Applications: WB IP IHC-P ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment (Human)
• Description: Rabbit Monoclonal
• Gene: ING2
• Conjugate: Unconjugated

Product images

ICC/IF image of ab109504 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109504 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

All lanes : Anti-ING2 antibody [EPR4597] (ab109504) at 1/10000 dilutionLane 1 : T47-D cell lysateLane 2 : HepG2 cell lysate Lane 3 : HT-1080 cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of ING2 expression in formalin-fixed, paraffin-embedded Human kidney tissue using ab109504 at 1/1000 dilution.