Anti-ING4 antibody (ab90551) at 1 µg/ml + A431 cell lysate at 50 µg
ING4 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Mouse polyclonal to ING4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab90551.Secondary: Protein G-HRP at 1/500 dilution.Band: 26kDa: ING4.
Anti-ING4 antibody (ab90551) at 1 µg/ml + NIH 3T3 cell lysate at 50 µg
All lanes : Anti-ING4 antibody (ab90551) at 1 µg/mlLane 1 : ING4-transfected 293T cell lysateLane 2 : non transfected lysateLysates/proteins at 25 µg per lane.
ab90551 at 3 µg/ml staining ING4 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry. Antigen retrieval with the microwave method was performed.