![All lanes : Anti-INSL3 antibody [EPR14834(2)] (ab199536) at 1/5000 dilutionLane 1 : Mouse testis lysateLane 2 : Human testis lysateLane 3 : Human fetal heart lysateLane 4 : Human fetal kidney lysateLane 5 : Human fetal spleen lysateLane 6 : NCCIT (Human pluripotent embryonal carcinoma) whole cell lysateLane 7 : A431 (Human epidermoid carcinoma) whole cell lysateLane 8 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/8783_ab199536-242166-ab199536WB.jpg)
All lanes : Anti-INSL3 antibody [EPR14834(2)] (ab199536) at 1/5000 dilutionLane 1 : Mouse testis lysateLane 2 : Human testis lysateLane 3 : Human fetal heart lysateLane 4 : Human fetal kidney lysateLane 5 : Human fetal spleen lysateLane 6 : NCCIT (Human pluripotent embryonal carcinoma) whole cell lysateLane 7 : A431 (Human epidermoid carcinoma) whole cell lysateLane 8 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

INSL3 was immunoprecipitated from 1mg of Human testis whole cell lysate with ab199536 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199536 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Human testis whole cell lysate 10 µg (Input). Lane 2: ab199536 IP in Human testis whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199536 in Human testis whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.