Anti-Insulin degrading enzyme / IDE antibody (ab63137) at 1/60 dilution + A375 cell line lysates at 35 µg
Immunohistochemical analysis of Insulin degrading enzyme / IDE expression in paraffin embedded formalin fixed human hepatocarcinoma tissue using 1/50 ab63137, followed by peroxidase-conjugated secondary antibody step and DAB staining.
ICC/IF image of ab63137 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63137, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.