Anti-Integrin alpha 2+beta 1 antibody [P1E6]
| Name | Anti-Integrin alpha 2+beta 1 antibody [P1E6] |
|---|---|
| Supplier | Abcam |
| Catalog | ab24697 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | P1E6 |
| Applications | IP B/N B/N FC B/N ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Tissue/ cell preparation (Human): HT1080 fibrosarcoma cells |
| Description | Mouse Monoclonal |
| Gene | ITGA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HT1080 cells stained with ab24697 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24697, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HT1080 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/8971_Integrin-alpha-2beta-1-Primary-antibodies-ab24697-7.jpg)
Overlay histogram showing HT1080 cells stained with ab24697 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24697, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HT1080 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
ICC/IF image of ab24697 stained HepG2 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24697, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Expression of estrogen receptor beta increases integrin alpha1 and integrin beta1 - Expression of estrogen receptor beta increases integrin alpha1 and integrin beta1
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Clustering of syndecan-4 and integrin beta1 by laminin alpha 3 chain-derived - Clustering of syndecan-4 and integrin beta1 by laminin alpha 3 chain-derived
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