Anti-Integrin alpha 5 antibody [P1D6]
| Name | Anti-Integrin alpha 5 antibody [P1D6] |
|---|---|
| Supplier | Abcam |
| Catalog | ab78614 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG3 |
| Clone | P1D6 |
| Applications | DB IHC-F FC B/N ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Tissue, cells or virus corresponding to Human Integrin alpha 5 |
| Description | Mouse Monoclonal |
| Gene | ITGA5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab78614 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine (ab120050) to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78614, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed HeLa cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/9035_Integrin-alpha-5-Primary-antibodies-ab78614-1.jpg)
Overlay histogram showing HeLa cells stained with ab78614 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine (ab120050) to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78614, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed HeLa cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Flow Cytometry Analysis: Representative lot data.Cell surface staining of HT-1080 cells using 1 µg/mL of ab78614 (shaded histogram) and an isotype control (unshaded histogram). Cells were detected using a mouse IgG-FITC.
Product References
A novel cell adhesion region in tropoelastin mediates attachment to integrin - A novel cell adhesion region in tropoelastin mediates attachment to integrin
Lee P, Bax DV, Bilek MM, Weiss AS. J Biol Chem. 2014 Jan 17;289(3):1467-77.
Design of a novel fibronectin-mimetic peptide-amphiphile for functionalized - Design of a novel fibronectin-mimetic peptide-amphiphile for functionalized
Mardilovich A, Craig JA, McCammon MQ, Garg A, Kokkoli E. Langmuir. 2006 Mar 28;22(7):3259-64.