All lanes : Anti-Integrin beta 4 antibody [EPR8559] (ab133682) at 1/1000 dilutionLane 1 : BxPC-3 cell lysateLane 2 : SW480 cell lysateLysates/proteins at 10 µg/ml per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
ab133682 staining Integrin beta 4 in human breast epithelail cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.See Abreview
Immunofluorescent analysis of BxPC-3 cells labelling Integrin beta 4 with ab133682 at 1/250 dilution.
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD