Anti-Integrin beta 4 antibody [M126]
| Name | Anti-Integrin beta 4 antibody [M126] |
|---|---|
| Supplier | Abcam |
| Catalog | ab29042 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | M126 |
| Applications | ELISA WB ICC/IF FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Dog, Human |
| Antigen | recombinant protein containing amino acid residues in the cytoplasmic region of human integrin beta 4 |
| Description | Mouse Monoclonal |
| Gene | ITGB4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence analysis of rat 804G cells, staining Integrin beta 4 with ab29042.Cells were fixed in 4% formaldehyde in PBS for 8 minutes, washed thoroughly in PBS, and permeabilized in 0.1% Triton X-100 in PBS for 10 minutes. Samples were incubated with primary antibody at 37°C for 1 hour. The cells on coverslips were washed with PBS, and AlexaFluor®597-conjugated secondary antibodies were applied for 1 hour at 37°C.
![All lanes : Anti-Integrin beta 4 antibody [M126] (ab29042)Lane 1 : A431 (25 µg/lane) serum starved overnightLane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/9310_ab29042_1.jpg)
All lanes : Anti-Integrin beta 4 antibody [M126] (ab29042)Lane 1 : A431 (25 µg/lane) serum starved overnightLane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min
![Overlay histogram showing A431 cells stained with ab29042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29042, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/9311_ab29042-3-ab29042FC.jpg)
Overlay histogram showing A431 cells stained with ab29042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29042, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
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Xiang Y, Zhou XY, Tan YR, Tan ML, Liu HJ, Liu C, Qu XP, Qin XQ. PLoS One. 2014 Apr 16;9(4):e95533.
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Sparrow N, Manetti ME, Bott M, Fabianac T, Petrilli A, Bates ML, Bunge MB, Lambert S, Fernandez-Valle C. J Neurosci. 2012 Apr 11;32(15):5284-97.
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Klammer M, Kaminski M, Zedler A, Oppermann F, Blencke S, Marx S, Muller S, Tebbe A, Godl K, Schaab C. Mol Cell Proteomics. 2012 Sep;11(9):651-68.
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Wei W, Barron PD, Rheinwald JG. In Vitro Cell Dev Biol Anim. 2010 Dec;46(10):841-55. doi:
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Doehn U, Hauge C, Frank SR, Jensen CJ, Duda K, Nielsen JV, Cohen MS, Johansen JV, Winther BR, Lund LR, Winther O, Taunton J, Hansen SH, Frodin M. Mol Cell. 2009 Aug 28;35(4):511-22.