Overlay histogram showing Jurkat T cells stained with ab137058 (red line). Cells were incubated with the antibody at 1/100 in DMEM at 20°C for 30 minutes.The secondary antibody used was DyLight® 649 goat anti-rabbit IgG at 1/300 dilution. A non-specific IgG antibody was used for an isotype control (black line).See Abreview
Anti-Integrin beta 7 antibody [EP5948] (ab137058) at 1/1000 dilution + IM-9 cell lysate at 10 µgSecondaryHRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Immunofluorescent staining of IM-9 cells labelling Integrin beta 7 with ab137058 at 1/250 dilution.