Anti-Ionotropic Glutamate receptor 2 antibody

• Supplier: Abcam
• Catalog: ab20673
• Prices: $382.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F WB
• Species Reactivities: Mouse, Rat, Chicken, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GluR2
• Blocking Peptide: Mouse Ionotropic Glutamate receptor 2 peptide
• Description: Rabbit Polyclonal
• Gene: GRIA2
• Conjugate: Unconjugated

Product images

Lane 1 : MarkerLanes 2 - 5 : Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/mlLane 1 : As aboveLane 2 : Brain (Mouse) Tissue Lysate at 10 µgLane 3 : Cortex (Mouse) Tissue Lysate at 10 µgLane 4 : Brain (Mouse) Tissue Lysate at 10 µg with Mouse Ionotropic Glutamate receptor 2 peptide (ab25708) at 1 µg/mlLane 5 : Cortex (Mouse) Tissue Lysate at 10 µg with Mouse Ionotropic Glutamate receptor 2 peptide (ab25708) at 1 µg/mlSecondaryLanes 2 - 5 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Immunohistochemistical detection of Ionotropic Glutamate receptor 2 using antibody (ab20673) on PFA perfusion-fixed frozen rat brain sections. Primary antibody was used at 1/300, incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). The image demonstrates immunostaining obtained in the rat cortex, thin and punctuated cytoplasmic staining is observed as previously described for the hippocampus (J neurosci 2003 Nov 1923(33):10521-30). There is some staining in cellular processes as well (arrows).See Abreview

Product References

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