Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032]

• Supplier: Abcam
• Catalog: ab133477
• Prices: $381.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5032
• Applications: IP WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Ionotropic Glutamate receptor 2 aa 150-250
• Description: Rabbit Monoclonal
• Gene: GRIA2
• Conjugate: Unconjugated

Product images

ab133477 staining GluR2 in SK-N-SH cells treated with VU 0360223 (ab120803), by ICC/IF. Decrease of GluR2 expression correlates with increased concentration of VU 0360223, as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ab120803 (VU 0360223) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab133477 (1/200 dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477) at 1/1000 dilutionLane 1 : Fetal brain lysateLane 2 : Human brain lysateLane 3 : Human hippocampus lysateLane 4 : Human hypothalamus lysateLane 5 : Human midbrain lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Product References

Synapse elimination and learning rules co-regulated by MHC class I H2-Db. - Synapse elimination and learning rules co-regulated by MHC class I H2-Db.

Lee H, Brott BK, Kirkby LA, Adelson JD, Cheng S, Feller MB, Datwani A, Shatz CJ. Nature. 2014 May 8;509(7499):195-200.

Hypofunction of glutamatergic neurotransmission in the periaqueductal gray - Hypofunction of glutamatergic neurotransmission in the periaqueductal gray

Ho YC, Cheng JK, Chiou LC. J Neurosci. 2013 May 1;33(18):7825-36.