
Western blot analysis of IRAK4 in HeLa cell lysate with ab5985 at 1 (lane 1), 2 (lane 2), and 4 (lane 3) µg/ml, respectively.

ab5985 at 10µg/ml staining IRAK-4 in K562 by ICC/IF

ab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568.Left image: DAPI staining nuclei (blue)Middle image: IRAK4 (red)Right image: OverlaySee Abreview

Immunofluorescence of IRAK-4 in K562 cells using ab5985 at 10 ug/ml.