IRAK4

Gene Symbol IRAK4
Entrez Gene 51135
Alt Symbol IPD1, IRAK-4, NY-REN-64, REN64
Species Human
Gene Type protein-coding
Description interleukin-1 receptor-associated kinase 4
Other Description renal carcinoma antigen NY-REN-64
Swissprots Q9Y589 Q9NWZ3 Q8TDF7 Q69FE1
Accessions AAN75440 CAD37279 EAW57867 Q9NWZ3 AF155118 AAD42884 AF445802 AAM15772 AI126481 AK000528 BAA91232 AK027301 AK299944 BAG61774 AK304583 BAG65371 AK314288 BAG36946 AY283670 AAP57089 AY283671 AAP57090 AY340962 AAR02358 AY340963 AAR02359 AY340964 AAR02360 AY340965 AAR02361 AY340966 AAR02362 AY340967 AAR02363 AY730682 AAX22228 BC013316 AAH13316 DA290809 DQ892885 ABM83811 DQ896134 ABM87133 LK938723 XM_005268943 XP_005269000 XM_005268944 XP_005269001 XM_005268945 XP_005269002 XM_005268946 XP_005269003 XM_005268947 XP_005269004 XM_005268948 XP_005269005 XM_005268949 XP_005269006 XM_006719438 XP_006719501 XM_006719439 XP_006719502 XM_006719440 XP_006719503 XM_006719441 XP_006719504 XM_006719442 XP_006719505 XM_006719443 XP_006719506 XM_011538431 XP_011536733 XM_011538432 XP_011536734 XM_011538433 XP_011536735 NM_001114182 NP_001107654 NM_001145256 NP_001138728 NM_001145257 NP_001138729 NM_001145258 NP_001138730 NM_016123 NP_057207
Function Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor- signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase a
Subcellular Location Cytoplasm {ECO:0000269|PubMed:21325272}.
Top Pathways Toxoplasmosis, Tuberculosis, Influenza A, Measles, Pertussis

Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb (PE Conjugate) - 12914 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D6D7
Flow cytometric analysis of KARPAS-299 cells, untreated (blue) or treated with Human Interleukin-1β (hIL-1β) #8900 (50 ng/ml, 15 min) (green), using Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb (PE Conjugate). Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb - 11927 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6D7
Western blot analysis of serum-starved KARPAS-299 cell extracts, untreated (-) or treated with Human Interleukin-1β (hIL-1β) #8900 (50 ng/ml, 15 min; +), using Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb (upper) or IRAK4 Antibody #4363 (lower). Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

IRAK4 Antibody - 4363 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from THP-1 (human), RAW 264.7 (mouse), and H-4-II-E (rat) cell lines, using IRAK4 Antibody.

Phospho-IRAK4 (Thr345/Ser346) Antibody - 7652 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

IRAK-4 (G-2) - sc-374349 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IRAK-4 (G-2): sc-374349. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

IRAK-4 (H-100) - sc-99154 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
IRAK-4 (H-100) : sc-99154. Western blot analysis of IRAK-4 expression in Jurkat whole cell lysate.

IRAK-4 (S-20) - sc-34469 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

IRAK-4 (T-16) - sc-34470 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

IRAK-4 (5-RY38) - sc-134368 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
IRAK-4 (5-RY38): sc-134368. Western blot analysis of IRAK-4 expression in K-562 whole cell lysate.

Anti-IRAK4 antibody - ab5985 from Abcam

Prices $388.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of IRAK4 in HeLa cell lysate with ab5985 at 1 (lane 1), 2 (lane 2), and 4 (lane 3) µg/ml, respectively.

Anti-IRAK4 antibody - ab88124 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-IRAK4 antibody (ab88124) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µgSecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

Anti-IRAK4 antibody - ab188599 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-IRAK4 antibody (ab188599) at 1/500 dilution + NIH 3T3 cell lysate at 30 µg

Anti-IRAK4 antibody - ab109335 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab109335 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109335 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IRAK4 antibody - ab32898 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-IRAK4 antibody (ab32898) at 1 µg/ml (1 hour) + 35 ug of HeLa cell lysate in RIPA bufferdeveloped using the ECL technique

Anti-IRAK4 antibody - ab154271 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-IRAK4 antibody (ab154271) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

Anti-IRAK4 antibody - ab13685 from Abcam

Prices $378.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of IRAK-4 in 30 ugs of NIH 3T3 cell lysate using ab13685 at 1:500 dilution.

Anti-IRAK4 antibody [2H9] - ab119942 from Abcam

Prices $387.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2H9
Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilution + Recombinant IRAK4 protein

Anti-IRAK4 antibody [Y279] - ab32511 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y279
IHC image of ab32511 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32511, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-IRAK4 antibody - C-terminal - ab189108 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labelling IRAK4 with ab189108 at 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-IRAK4 antibody - N-terminal - ab71668 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-IRAK4 antibody - N-terminal (ab71668) at 1/100 dilution + Jurkat cell lysate at 12.5 µgSecondaryHRP-anti-rabbit

Anti-IRAK4 / IRAK-4 Antibody (N-Terminus) - LS-C8255 from LifeSpan Bioscience

Prices $495.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-IRAK4 / IRAK-4 Antibody (aa448-460) - LS-C8253 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-IRAK4 / IRAK-4 Antibody (aa436-459) - LS-C8256 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-IRAK4 / IRAK-4 Antibody (C-Terminus) - LS-C83586 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-IRAK4 / IRAK-4 Antibody (C-Terminus) - LS-C125828 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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