Anti-IRAK4 antibody [2H9]

• Supplier: Abcam
• Catalog: ab119942
• Prices: $387.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 2H9
• Applications: WB ELISA IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Human, Monkey
• Antigen: Recombinant fragment, corresponding to amino acids 21-198 of Human IRAK4
• Description: Mouse Monoclonal
• Gene: IRAK4
• Conjugate: Unconjugated

Product images

Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilution + Recombinant IRAK4 protein

All lanes : Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilutionLane 1 : THP-1 cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : MCF7 cell lysateLane 5 : RAW264.7 cell lysateLane 6 : Jurkat cell lysateLane 7 : COS7 cell lysate

ab119942, at 1/200 dilution, staining IRAK4 in paraffin-embedded Human lung cancer tissue by Immunohistochemistry with DAB staining.

ab119942, at 1/200 dilution, staining IRAK4 in paraffin-embedded Human kidney cancer tissue by Immunohistochemistry with DAB staining.

Flow cytometric analysis of Hela cells using ab119942 at 1/200 dilution (blue) and negative control (red).

ELISA using ab119942.

ab119942 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab119942 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.