IHC image of ab32511 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32511, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Anti-IRAK4 antibody [Y279] (ab32511) at 1/500 dilution + Jurkat cell lysateObserved band size : 52 kDa (why is the actual band size different from the predicted?)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/9876_ab32511wb.gif)
Anti-IRAK4 antibody [Y279] (ab32511) at 1/500 dilution + Jurkat cell lysateObserved band size : 52 kDa (why is the actual band size different from the predicted?)