Anti-IRAKM antibody - Carboxyterminal end

• Supplier: Abcam
• Catalog: ab8116
• Prices: $380.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Mouse, Rat, Sheep, Human
• Antigen: Synthetic peptide: CSSKFSWDEYEQYKKE , corresponding to C terminal amino acids 581-596 of Human IRAKM (Peptide available as ab6231
• Description: Rabbit Polyclonal
• Gene: IRAK3
• Conjugate: Unconjugated

Product images

All lanes : Anti-IRAKM antibody - Carboxyterminal end (ab8116) at 1 µg/mlLane 1 : Mouse spleen tissue lysatesLane 2 : Rat liver tissue lysateObserved band size : 68 kDa (why is the actual band size different from the predicted?)

ab8116 at 2µg/ml staining IRAKM in rat liver cells by IHC  

ab8116 staining IRAKM in murine lung macrophages by Immunocytochemistry/ Immunofluorescence.<.br>The cells were permeablized with a 1:1 mixture of methanol and acetone. After washing with PBS, ab8116 was applied and the cells incubated for one hour. After washing, antibodies against the primary antibody labeled with FITC were applied to the cells and incubated for one hour. The cell were then mounted in DAPI mounting media and viewed on the fluorescent microscope.

Immunofluorescence of IRAK-M in Rat Liver cells using ab8116 at 10 ug/ml.

Product References

TLR signaling prevents hyperoxia-induced lung injury by protecting the alveolar - TLR signaling prevents hyperoxia-induced lung injury by protecting the alveolar

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Mechanical ventilation modulates TLR4 and IRAK-3 in a non-infectious, - Mechanical ventilation modulates TLR4 and IRAK-3 in a non-infectious,

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IRAK-M regulates chromatin remodeling in lung macrophages during experimental - IRAK-M regulates chromatin remodeling in lung macrophages during experimental

Lyn-Kew K, Rich E, Zeng X, Wen H, Kunkel SL, Newstead MW, Bhan U, Standiford TJ. PLoS One. 2010 Jun 16;5(6):e11145.

Pulmonary and systemic endotoxin tolerance in preterm fetal sheep exposed to - Pulmonary and systemic endotoxin tolerance in preterm fetal sheep exposed to

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Chronic stimulation of Nod2 mediates tolerance to bacterial products. - Chronic stimulation of Nod2 mediates tolerance to bacterial products.

Hedl M, Li J, Cho JH, Abraham C. Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19440-5. Epub 2007 Nov 21.

Epithelial NF-kappaB activation promotes urethane-induced lung carcinogenesis. - Epithelial NF-kappaB activation promotes urethane-induced lung carcinogenesis.

Stathopoulos GT, Sherrill TP, Cheng DS, Scoggins RM, Han W, Polosukhin VV, Connelly L, Yull FE, Fingleton B, Blackwell TS. Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18514-9. Epub 2007 Nov 13.